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PSK植物脅迫測量套件

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北京澳作生態儀器有限公司

美國

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產品介紹

應用

Y(II)或ΔF/FM’ 或 (FM’ – FS )/FM’) 是經受時間考驗的光適應測量參數,比FV/FM對更多類型的植物脅迫更加敏感。已有的大量證據表明FV/FM對許多種植物脅迫和健康植物的光系統II的測量十分出色,而Y(II)或光量子產額則可測量實際光照下光適應環境和生理狀況的光系統II的效率。

原理

采用調制飽和脈沖原理,測量植物的葉綠素熒光,通過相關文獻的研究成果,計算植物的光量子產額及相對電子傳遞速率,同時可測量PAR、葉溫、相對濕度等環境參數。

特點

葉片吸收測量:提供葉片吸收測量及隨環境變化導致的葉片吸收變化。根據Eichelman (2004) 葉片吸收在健康植物的變化范圍在0.7~0.9 之間。因此,為獲得準確的ETR或“J”,Y(II)測量儀提供了一個可靠的測量方法,

FV/FM測量單元:可額外選配FV/FM測量儀,用于暗適應測量。

具有暗適應葉夾

陽光下屏幕可見

圖形顯示FV/FM曲線

2GB存儲空間

USB通訊

數據Excel查看

先進的PAR葉夾:采用底部葉夾打開裝置,防止測量時誤操作打開葉夾。對傳感器進行余弦校正,確保葉片相對測量光的角度不變。

FM’校正:對于具有高光照強度歷史的植物,完全關閉光反應中心是一個問題,Y(II)測量儀使用Loriaux &Genty 2013的方法進行FM’校正,確保誤差*小。

自動調制光設定:快速準確自動的調整合適的調制光強,避免人工操作的誤差。

先進算法避免飽和脈沖NPQ:采用25ms內8點的平均值確定FM’,消除飽和脈沖NPQ的影響。

更精確的葉溫測量:采用非接觸式紅外測量,測量精度可達±0.5℃。

直接測量相對濕度:含有測量氣體交換使用的固態傳感器,可測量相對濕度。

降低葉片遮擋的設計:傾斜的角度減少對葉片的遮擋,可以測量擬南芥等小葉。

系統組成

標配:

Y(II)光量子產額測量儀、充電器、USB電纜、便攜箱、2個吸收測量單元、U盤(包含說明書)。

可選:

FV/FM測量儀及10個暗適應葉夾、三腳架。

技術指標

測量參數

Y(II)或ΔF/Fm‘、ETR、PAR、T、FMS或FM’、Fs、α(葉片吸收)。

監測模式:可使用電腦,長時間監測Y(II)、ETR、葉片吸收、PAR、葉溫、相對濕度、及計算NPQ。

相對濕度:5%~95%,±2%。

可選參數:FV/FM、FV/FO,FO, FM, FV

可使用AC或USB供電,可配三腳架。

技術參數

光源

飽和脈沖:白色LED具有PAR時7000μmols

調制光:紅色LED 660nm,具有690nm短波過濾。

光化光源:僅可使用外部光源

檢測方法:調制脈沖法

檢測器&過濾器:具有700~750nm帶通過濾的PIN光電二極管

取樣速率:1~10000點/秒自動切換。

測量時間:3s或長期監測

存儲空間:2GB

輸出:USB

尺寸:便攜箱尺寸為14”x 11”x 6”,儀器為9’’長

質量:Y(II) 測量儀0.45 kg

FV/FM測量儀0.36 kg.

總重1.95 kg.

產地

美國

文獻

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Adams WW III, Demmig-Adams B. (1994) Carotenoid composition and down regulation of Photosystem II in three conifer species during the winter. Physiol Plant 92: 451-458

Ball MC. (1994) The role of photoinhibition during seedling establishment at low temperatures. In: Baker NR. And Bowyer JR. (eds) Photoinhibition of Photosynthesis. From Molecular Mechanisms to the Field, pp365-3376 Bios Scientific Publishers, Oxford

Ball MC., Butterworth JA., Roden JS., Christian R., Egerton JJG., (1995) Applications of chlorophyll fluorescence to forest ecology. Aust. J. Plant Physiology 22: 311-319

Baker N.R, Rosenquist E. (2004) Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities, Bukhov & Carpentier 2004 – Effects of Water Stress on the Photosynthetic Efficiency of Plants, Bukhov NG., & Robert Carpentier, From Chapter 24, “Chlorophyll a Fluorescence a Signature of Photosynthesis”, edited by George

Papaqeorgiou and Govindjee, published by Springer 2004, PO Box 17, 3300 AA Dordrecht, The Netherlands, page 627-628 Burke J. (2007) Evaluation of Source Leaf Responses to Water-Deficit Stresses in Cotton Using a Novel Stress Bioassay, Plant Physiology, Jan. 2007, Vol 143, pp108-121

Burke J., Franks C.D. Burow G., Xin Z. (2010) Selection system for the Stay-Green Drought Tolerance Trait in Sorghum Germplasm, Agronomy Journal 102:1118-1122 May 2010

Cavender-Bares J. & Fakhri A. Bazzaz 2004 – “From Leaves to Ecosystem: Using Chlorophyll Fluorescence to Assess Photosynthesis and Plant Function in Ecological Studies”. Jeannine Cavender Bares, Fakhri A. Bazzaz, From Chapter 29, “Chlorophyll a Fluorescence a Signature of Photosynthesis”, edited by George Papaqeorgiou and Govindjee, published by Springer 2004, PO Box 17, 3300 AA Dordrecht, The Netherlands, page 746-747 ETR Drought stress and npq

Cazzaniga S, Osto L.D., Kong S-G., Wada M., Bassi R., (2013) “Interaction between avoidance of photon absorption, excess energy dissipation and zeaxanthin synthesis against photo oxidative stress in Arabidopsis”, The Plant Journal, Volume 76, Issue 4, pages568–579, November 2013 DOI: 10.1111/tpj.12314

Cheng L., Fuchigami L., Breen P., (2001) “The relationship between photosystem II efficiency and quantum yield for CO2 assimilation is not affected by nitrogen content in apple leaves.”

Adams WW III, Demmig-Adams B., Vernhoeven AS., and Barker DH., (1995) Photoinhibition during winter stress – Involvement of sustained xanthophyll cycle-dependent energy-dissipation. Aust J. Plant Physiol 22: 261-276 Journal of Experimental Botany, 55(403):1607-1621

Journal of Experimental Botany, 52(362):1865-1872Crafts-Brandner S. J., Law R.D. (2000) Effects of heat stress on the inhibition and recovery of ribulase-1, 5- biphsphate carboxylase/ oxygenase activation state. Planta (2000) 212: 67-74

all’Osto L, Cazzaniga S, Wada M, Bassi R. (2014) On the origin of a slowly reversible fluorescence decay component in the Arabidopsis npq4 mutant. Phil. Trans. R. Soc. B 369: 20130221.

da Silva J. A. & Arrabaca M.C. (2008).Physiologia Plantarum Volume 121 Issue 3, Pages 409 – 420 2008

Eichelman H., Oja V., Rasulov B., Padu E., Bichele I., Pettai H., Niinemets O., Laisk A. (2004) Development of Leaf Photosynthetic Parameters in Betual pendula Roth Leaves: Correlation with Photosystem I Density, Plant Biology 6 (2004):307-318

Eyodogan F., Oz M. T. (2007) Effect of salinity on antioxidant responses of chickpea seedlings. Acta Physiol Plant (2007) 29:485-493

Flexas 1999 – “Water stress induces different levels of photosynthesis and electron transport rate regulation in grapevines”J. FLEXAS, J. M. ESCALONA & H. MEDRANO Plant, Cell & Environment Volume 22 Issue 1 Page 39-48, January 1999

Flexas 2000 – “Steady-State and Maximum Chlorophyll Fluorescence Responses to Water Stress In Grape Vine Leaves: A New Remote Sensing System”, J. Flexas, MJ Briantais, Z Cerovic, H Medrano, I Moya, Remote Sensing Environment 73:283-270 Physiologia Plantarum, Volume 114, Number 2, February 2002 , pp. 231-240(10)

Gonias E. D. Oosterhuis D.M., Bibi A.C. & Brown R.S. (2003) YIELD, GROWTH AND PHYSIOLOGY OF TRIMAX TM TREATED COTTON, Summaries of Arkansas Cotton Research 2003

Hendrickson L., Furbank R., & Chow (2004) A simple alternative approach to assessing the fate of absorbed Light energy using chlorophyll fluorescence. Photosynthesis Research 82: 73-81

Kramer D. M., Johnson G., Kiirats O., Edwards G. (2004) New fluorescence parameters for determination of QA redox state and excitation energy fluxes. Photosynthesis Research 79: 209-218

Krause G.H., Weis E. (1984) Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. 5, 139-157.

Krupa Z., Oquist G., and Huner N., (1993) The effects of cadmium on photosynthesis of Phaseolus vulgaris – a fluorescence analysis. Physiol Plant 88, 626-630

D Edwards GE and Baker NR (1993) Can CO2 assimilation in maize leaves be predicted accurately from chlorophyll fluorescence analysis? Photosynth Res 37: 89–102

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Photosynthesis in the water-stressed C grass is mainly limited by stomata with both rapidly and slowly imposed water deficits. Flexas (2002) Steady-state chlorophyll fluorescence (Fs) measurements as a tool to follow variations of net CO2 assimilation and stomatal conductance during water-stress in C plants Flexas J., Escalona J. M., Evain S., Gulías J., Moya I., Charles Barry Osmond C.B., and Medrano H. 4 Setaria sphacelata

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Loriaux S.D., R.A Burns,Welles J.M., McDermitt D.K. Genty B. (2006) “Determination of Maximal Chlorophyll Fluorescence Using A Multiphase Single Flash of Sub-Saturating Intensity”. Abstract # P13011 August 1996.

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