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>快速動力學(xué)停流裝置與光譜分析系統(tǒng)(Stopped-Flow)

快速動力學(xué)停流裝置與光譜分析系統(tǒng)(Stopped-Flow)

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1100

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儀器簡介:快速動力學(xué)停流裝置與光譜儀器配合進行快速動力學(xué)瞬間實時記錄,或離線分析,為廣大的快速動力學(xué)研究學(xué)者提供了強有力的武器。選擇不同的配置可進行下列應(yīng)用:

* 蛋白質(zhì)折疊動力學(xué)(Protein Folding)

* 酶動力學(xué)(Enzyme Kinetice)

* 底物結(jié)合(Substrate Binding)

* 構(gòu)象轉(zhuǎn)變(Conformational Changes)

* 胞內(nèi)底物運輸(Substrate Transport in Vesicles)

* 第二信使研究(Second Messenger Studies)

* 快速化學(xué)反應(yīng)動力學(xué)(Rapid Chemical Reaction Kinetics)

KinTek has always been aleader in building stopped-flow instrumentsthat conserve precious samples. The all new AutoSF-120 sets a new standard for sample economy, requiringonly 120 microliters of sample to flush, load and collect data, while giving with 3-4 replicate measurements.The flushing and sample loading areall done automatically. Simply pipette 120 microliters of solution into each sample cup and click "Load".

In the process of designing this new state-of-the-art instrument, we also added new noise reduction electronic circuits to improve signal:noise twofold and achieved asubmillisecond dead time. In addition we completely re-wrote the system software for data collection and analysis to give unparalleled ease of use and power.

The KinTek AutoSF is by far the best stopped-flow instrument ever built, giving you more high quality data while consumingless sample and less time.

An optionalrobotic autosamplerincreases throughput even more to afford unattended stopped-flow measurements ofhundreds of samples per day.

技術(shù)參數(shù):

1.注射器數(shù)量:3個

2.驅(qū)動方式:每一個注射器由一個步進馬達(dá)驅(qū)動

3.*小死時間:0.6毫秒(FC-08池)0.25毫秒(微量池)

4.注射流量范圍:0.01-10ml/s(取決于安裝的注射器)

5.混合比例范圍:1/1至1/100連續(xù)可調(diào)

主要特點:

1.恒溫水夾套設(shè)計,全電腦控制操作

2.程控驅(qū)動注射器,每路樣品流量可調(diào),不受濃度與粘度影響

3.高精度,完全可調(diào)的混合與稀釋比例

4.極短的死時間,*短為0.25毫秒

5.二路、三路、四路流動注射停流裝置可選擇

  • Small sample volume:120 μL is sufficientto flush and load a new sample and get3-4 reproducible shots.All other instruments require 3-5 times as much sample.

  • Automated computer-controlledload/flush/fire cycle — simply pipet 120 μL into each sample cup and click "load".

  • Twofold improvement in signal/noise with new noise reduction circuits

  • Compact footprint: instrument and electronics combined (14 × 26.5 × 4.5 inches)

  • Computer optimized optical path with lower noise and background fluorescence

  • Automated sample loader, which can be interfaced with an optional robot.

  • 200 μl of sample is sufficient to load and collect 6-8 good shots.

  • Standard 10 μl flow cell.

  • 20 μl per shot per solution.

  • Hastwo fluorescence channelsandone absorbance channelthat can be observed and recorded simultaneously.

  • Under complete computer control, courtesy of the KinTek StopFlow Software.

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